Review



lk1108 single cell suspensions  (Corning Life Sciences)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Corning Life Sciences lk1108 single cell suspensions
    Comparative drug response profiles of 2D cell cultures and MCSs. ( a – c ) Drug response curves for LK0917, LK0902, and <t>LK1108</t> for 2D and MCSs cultures treated with 170–17000 µM of doxorubicin; 3.33–333 µM of cisplatin; and 2.2–220 µM of methotrexate. ( d ) IC 50 (µM) values calculated from the drug response curves for both 2D and MCSs of all cell types for all drugs. Significantly large differences between the IC 50 values of 2D and MCSs are denoted with “ # ”. Each IC 50 value is the average of three independent experiments (n = 3) with triplicates set for each concentration.
    Lk1108 Single Cell Suspensions, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lk1108 single cell suspensions/product/Corning Life Sciences
    Average 90 stars, based on 1 article reviews
    lk1108 single cell suspensions - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Dissecting multi drug resistance in head and neck cancer cells using multicellular tumor spheroids"

    Article Title: Dissecting multi drug resistance in head and neck cancer cells using multicellular tumor spheroids

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-56273-6

    Comparative drug response profiles of 2D cell cultures and MCSs. ( a – c ) Drug response curves for LK0917, LK0902, and LK1108 for 2D and MCSs cultures treated with 170–17000 µM of doxorubicin; 3.33–333 µM of cisplatin; and 2.2–220 µM of methotrexate. ( d ) IC 50 (µM) values calculated from the drug response curves for both 2D and MCSs of all cell types for all drugs. Significantly large differences between the IC 50 values of 2D and MCSs are denoted with “ # ”. Each IC 50 value is the average of three independent experiments (n = 3) with triplicates set for each concentration.
    Figure Legend Snippet: Comparative drug response profiles of 2D cell cultures and MCSs. ( a – c ) Drug response curves for LK0917, LK0902, and LK1108 for 2D and MCSs cultures treated with 170–17000 µM of doxorubicin; 3.33–333 µM of cisplatin; and 2.2–220 µM of methotrexate. ( d ) IC 50 (µM) values calculated from the drug response curves for both 2D and MCSs of all cell types for all drugs. Significantly large differences between the IC 50 values of 2D and MCSs are denoted with “ # ”. Each IC 50 value is the average of three independent experiments (n = 3) with triplicates set for each concentration.

    Techniques Used: Concentration Assay

    Real-time monitoring of calcein-AM uptake in 2D and MCSs. ( a ) Elucidate calcein-AM uptake in 2D cell cultures and MCSs obtained from LK0917- MCS 17 (upper panel), LK0902- MCS 02 (middle panel), and LK1108- MCS 08 (lower panel) and over a time span of 10 hours with image acquisition at 20-minute intervals (scale bar, 200 μm). ( b ) Heat map pseudo color images of MCSs for differential calcein-AM uptake for the three cell lines (scale bar, 200 μm, scale bar on the right represents the pixel intensity of green fluorescence). ( c ) Mean fluorescence intensity profile with respect to time (12 hours) in the 2D cell cultures (LK0917, LK0902, and LK1108) and MCSs (MCS 17 , MCS 02 , and MCS 08 ) respectively. ( d ) Total accumulated calcein over time (12 hours) in 2D and MCSs for all three cell lines. ( e ) Total accumulated calcein profiles of MCSs obtained from different cell densities. The data are shown as mean ± SD; ***p < 0.001, **p < 0.05, *p = 0.019, and ns = non-significant (n = 3).
    Figure Legend Snippet: Real-time monitoring of calcein-AM uptake in 2D and MCSs. ( a ) Elucidate calcein-AM uptake in 2D cell cultures and MCSs obtained from LK0917- MCS 17 (upper panel), LK0902- MCS 02 (middle panel), and LK1108- MCS 08 (lower panel) and over a time span of 10 hours with image acquisition at 20-minute intervals (scale bar, 200 μm). ( b ) Heat map pseudo color images of MCSs for differential calcein-AM uptake for the three cell lines (scale bar, 200 μm, scale bar on the right represents the pixel intensity of green fluorescence). ( c ) Mean fluorescence intensity profile with respect to time (12 hours) in the 2D cell cultures (LK0917, LK0902, and LK1108) and MCSs (MCS 17 , MCS 02 , and MCS 08 ) respectively. ( d ) Total accumulated calcein over time (12 hours) in 2D and MCSs for all three cell lines. ( e ) Total accumulated calcein profiles of MCSs obtained from different cell densities. The data are shown as mean ± SD; ***p < 0.001, **p < 0.05, *p = 0.019, and ns = non-significant (n = 3).

    Techniques Used: Fluorescence

    ROS activity of all cell lines in 2D and MCSs. ( a ) Live-cell fluorescence images of the 2D (LK0917, LK0902, and LK1108) and MCSs (MCS 17 , MCS 02 , and MCS 08 ) cultures obtained from cell lines in the presence of DCFDA over a period of 60 minutes with image acquisition at 20-minute intervals (upper, middle and lower panel respectively), scale bar, 200 μm). ( b ) Redox state in 2D and MCSs cultures. Data are presented as the mean ± SD; ***p < 0.001, **p < 0.05, *p = 0.019, and no significant differences were observed in case of 2D (n = 3). ( c ) Heat map pseudo color images of MCSs for differential redox status for the three cell lines (scale bar, 200 μm).
    Figure Legend Snippet: ROS activity of all cell lines in 2D and MCSs. ( a ) Live-cell fluorescence images of the 2D (LK0917, LK0902, and LK1108) and MCSs (MCS 17 , MCS 02 , and MCS 08 ) cultures obtained from cell lines in the presence of DCFDA over a period of 60 minutes with image acquisition at 20-minute intervals (upper, middle and lower panel respectively), scale bar, 200 μm). ( b ) Redox state in 2D and MCSs cultures. Data are presented as the mean ± SD; ***p < 0.001, **p < 0.05, *p = 0.019, and no significant differences were observed in case of 2D (n = 3). ( c ) Heat map pseudo color images of MCSs for differential redox status for the three cell lines (scale bar, 200 μm).

    Techniques Used: Activity Assay, Fluorescence

    ( a –c) Median fluorescence intensity (MFI) values for 2D and MCSs cultures of LK0917, LK0902, and LK1108 in presence of different ABC pump inhibitors for MDR1, MRP1, and BCRP. ( d ) MFI for MDR1 pump inhibitor (verapamil) and its corresponding live cell imaging for 2D and MCSs cultures. ( e ) Total calcein-AM uptake for the entire course of 10 hours +/− verapamil for 2D and MCSs. The data are shown as a mean of ± SD, ***p < 0.001, **p < 0.05. ( f ) Elucidate calcein-AM uptake in 2D for LK0917, LK0902 and LK1108 in presence and absence of verapamil over a time span of 10 hours with images taken after every 20 minutes (images shown here are at 2 h interval). Scale bar 200 μm.
    Figure Legend Snippet: ( a –c) Median fluorescence intensity (MFI) values for 2D and MCSs cultures of LK0917, LK0902, and LK1108 in presence of different ABC pump inhibitors for MDR1, MRP1, and BCRP. ( d ) MFI for MDR1 pump inhibitor (verapamil) and its corresponding live cell imaging for 2D and MCSs cultures. ( e ) Total calcein-AM uptake for the entire course of 10 hours +/− verapamil for 2D and MCSs. The data are shown as a mean of ± SD, ***p < 0.001, **p < 0.05. ( f ) Elucidate calcein-AM uptake in 2D for LK0917, LK0902 and LK1108 in presence and absence of verapamil over a time span of 10 hours with images taken after every 20 minutes (images shown here are at 2 h interval). Scale bar 200 μm.

    Techniques Used: Fluorescence, Live Cell Imaging



    Similar Products

    lk1108 single cell suspensions  (Corning Life Sciences)
    90
    Corning Life Sciences lk1108 single cell suspensions
    Comparative drug response profiles of 2D cell cultures and MCSs. ( a – c ) Drug response curves for LK0917, LK0902, and <t>LK1108</t> for 2D and MCSs cultures treated with 170–17000 µM of doxorubicin; 3.33–333 µM of cisplatin; and 2.2–220 µM of methotrexate. ( d ) IC 50 (µM) values calculated from the drug response curves for both 2D and MCSs of all cell types for all drugs. Significantly large differences between the IC 50 values of 2D and MCSs are denoted with “ # ”. Each IC 50 value is the average of three independent experiments (n = 3) with triplicates set for each concentration.
    Lk1108 Single Cell Suspensions, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lk1108 single cell suspensions/product/Corning Life Sciences
    Average 90 stars, based on 1 article reviews
    lk1108 single cell suspensions - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Comparative drug response profiles of 2D cell cultures and MCSs. ( a – c ) Drug response curves for LK0917, LK0902, and LK1108 for 2D and MCSs cultures treated with 170–17000 µM of doxorubicin; 3.33–333 µM of cisplatin; and 2.2–220 µM of methotrexate. ( d ) IC 50 (µM) values calculated from the drug response curves for both 2D and MCSs of all cell types for all drugs. Significantly large differences between the IC 50 values of 2D and MCSs are denoted with “ # ”. Each IC 50 value is the average of three independent experiments (n = 3) with triplicates set for each concentration.

    Journal: Scientific Reports

    Article Title: Dissecting multi drug resistance in head and neck cancer cells using multicellular tumor spheroids

    doi: 10.1038/s41598-019-56273-6

    Figure Lengend Snippet: Comparative drug response profiles of 2D cell cultures and MCSs. ( a – c ) Drug response curves for LK0917, LK0902, and LK1108 for 2D and MCSs cultures treated with 170–17000 µM of doxorubicin; 3.33–333 µM of cisplatin; and 2.2–220 µM of methotrexate. ( d ) IC 50 (µM) values calculated from the drug response curves for both 2D and MCSs of all cell types for all drugs. Significantly large differences between the IC 50 values of 2D and MCSs are denoted with “ # ”. Each IC 50 value is the average of three independent experiments (n = 3) with triplicates set for each concentration.

    Article Snippet: For the generation of MCSs sized 300–500 μm, 200 μL of LK0917 (MCS 17 ), LK0902 (MCS 02 ), and LK1108 (MCS 08 ) single cell suspensions were seeded in ultra-low attachment (ULA) plates (Corning Life Sciences, Massachusetts, USA) at varying cell densities in the range of 0.25–0.75 × 10 5 cells/mL.

    Techniques: Concentration Assay

    Real-time monitoring of calcein-AM uptake in 2D and MCSs. ( a ) Elucidate calcein-AM uptake in 2D cell cultures and MCSs obtained from LK0917- MCS 17 (upper panel), LK0902- MCS 02 (middle panel), and LK1108- MCS 08 (lower panel) and over a time span of 10 hours with image acquisition at 20-minute intervals (scale bar, 200 μm). ( b ) Heat map pseudo color images of MCSs for differential calcein-AM uptake for the three cell lines (scale bar, 200 μm, scale bar on the right represents the pixel intensity of green fluorescence). ( c ) Mean fluorescence intensity profile with respect to time (12 hours) in the 2D cell cultures (LK0917, LK0902, and LK1108) and MCSs (MCS 17 , MCS 02 , and MCS 08 ) respectively. ( d ) Total accumulated calcein over time (12 hours) in 2D and MCSs for all three cell lines. ( e ) Total accumulated calcein profiles of MCSs obtained from different cell densities. The data are shown as mean ± SD; ***p < 0.001, **p < 0.05, *p = 0.019, and ns = non-significant (n = 3).

    Journal: Scientific Reports

    Article Title: Dissecting multi drug resistance in head and neck cancer cells using multicellular tumor spheroids

    doi: 10.1038/s41598-019-56273-6

    Figure Lengend Snippet: Real-time monitoring of calcein-AM uptake in 2D and MCSs. ( a ) Elucidate calcein-AM uptake in 2D cell cultures and MCSs obtained from LK0917- MCS 17 (upper panel), LK0902- MCS 02 (middle panel), and LK1108- MCS 08 (lower panel) and over a time span of 10 hours with image acquisition at 20-minute intervals (scale bar, 200 μm). ( b ) Heat map pseudo color images of MCSs for differential calcein-AM uptake for the three cell lines (scale bar, 200 μm, scale bar on the right represents the pixel intensity of green fluorescence). ( c ) Mean fluorescence intensity profile with respect to time (12 hours) in the 2D cell cultures (LK0917, LK0902, and LK1108) and MCSs (MCS 17 , MCS 02 , and MCS 08 ) respectively. ( d ) Total accumulated calcein over time (12 hours) in 2D and MCSs for all three cell lines. ( e ) Total accumulated calcein profiles of MCSs obtained from different cell densities. The data are shown as mean ± SD; ***p < 0.001, **p < 0.05, *p = 0.019, and ns = non-significant (n = 3).

    Article Snippet: For the generation of MCSs sized 300–500 μm, 200 μL of LK0917 (MCS 17 ), LK0902 (MCS 02 ), and LK1108 (MCS 08 ) single cell suspensions were seeded in ultra-low attachment (ULA) plates (Corning Life Sciences, Massachusetts, USA) at varying cell densities in the range of 0.25–0.75 × 10 5 cells/mL.

    Techniques: Fluorescence

    ROS activity of all cell lines in 2D and MCSs. ( a ) Live-cell fluorescence images of the 2D (LK0917, LK0902, and LK1108) and MCSs (MCS 17 , MCS 02 , and MCS 08 ) cultures obtained from cell lines in the presence of DCFDA over a period of 60 minutes with image acquisition at 20-minute intervals (upper, middle and lower panel respectively), scale bar, 200 μm). ( b ) Redox state in 2D and MCSs cultures. Data are presented as the mean ± SD; ***p < 0.001, **p < 0.05, *p = 0.019, and no significant differences were observed in case of 2D (n = 3). ( c ) Heat map pseudo color images of MCSs for differential redox status for the three cell lines (scale bar, 200 μm).

    Journal: Scientific Reports

    Article Title: Dissecting multi drug resistance in head and neck cancer cells using multicellular tumor spheroids

    doi: 10.1038/s41598-019-56273-6

    Figure Lengend Snippet: ROS activity of all cell lines in 2D and MCSs. ( a ) Live-cell fluorescence images of the 2D (LK0917, LK0902, and LK1108) and MCSs (MCS 17 , MCS 02 , and MCS 08 ) cultures obtained from cell lines in the presence of DCFDA over a period of 60 minutes with image acquisition at 20-minute intervals (upper, middle and lower panel respectively), scale bar, 200 μm). ( b ) Redox state in 2D and MCSs cultures. Data are presented as the mean ± SD; ***p < 0.001, **p < 0.05, *p = 0.019, and no significant differences were observed in case of 2D (n = 3). ( c ) Heat map pseudo color images of MCSs for differential redox status for the three cell lines (scale bar, 200 μm).

    Article Snippet: For the generation of MCSs sized 300–500 μm, 200 μL of LK0917 (MCS 17 ), LK0902 (MCS 02 ), and LK1108 (MCS 08 ) single cell suspensions were seeded in ultra-low attachment (ULA) plates (Corning Life Sciences, Massachusetts, USA) at varying cell densities in the range of 0.25–0.75 × 10 5 cells/mL.

    Techniques: Activity Assay, Fluorescence

    ( a –c) Median fluorescence intensity (MFI) values for 2D and MCSs cultures of LK0917, LK0902, and LK1108 in presence of different ABC pump inhibitors for MDR1, MRP1, and BCRP. ( d ) MFI for MDR1 pump inhibitor (verapamil) and its corresponding live cell imaging for 2D and MCSs cultures. ( e ) Total calcein-AM uptake for the entire course of 10 hours +/− verapamil for 2D and MCSs. The data are shown as a mean of ± SD, ***p < 0.001, **p < 0.05. ( f ) Elucidate calcein-AM uptake in 2D for LK0917, LK0902 and LK1108 in presence and absence of verapamil over a time span of 10 hours with images taken after every 20 minutes (images shown here are at 2 h interval). Scale bar 200 μm.

    Journal: Scientific Reports

    Article Title: Dissecting multi drug resistance in head and neck cancer cells using multicellular tumor spheroids

    doi: 10.1038/s41598-019-56273-6

    Figure Lengend Snippet: ( a –c) Median fluorescence intensity (MFI) values for 2D and MCSs cultures of LK0917, LK0902, and LK1108 in presence of different ABC pump inhibitors for MDR1, MRP1, and BCRP. ( d ) MFI for MDR1 pump inhibitor (verapamil) and its corresponding live cell imaging for 2D and MCSs cultures. ( e ) Total calcein-AM uptake for the entire course of 10 hours +/− verapamil for 2D and MCSs. The data are shown as a mean of ± SD, ***p < 0.001, **p < 0.05. ( f ) Elucidate calcein-AM uptake in 2D for LK0917, LK0902 and LK1108 in presence and absence of verapamil over a time span of 10 hours with images taken after every 20 minutes (images shown here are at 2 h interval). Scale bar 200 μm.

    Article Snippet: For the generation of MCSs sized 300–500 μm, 200 μL of LK0917 (MCS 17 ), LK0902 (MCS 02 ), and LK1108 (MCS 08 ) single cell suspensions were seeded in ultra-low attachment (ULA) plates (Corning Life Sciences, Massachusetts, USA) at varying cell densities in the range of 0.25–0.75 × 10 5 cells/mL.

    Techniques: Fluorescence, Live Cell Imaging